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The Five Constant Relationships In The Confucian Approach - Colorimetric Analysis This will depend on the colorimeter you have available to work with in your school. Your teacher should be able to guide you, but you need to make sure you are measuring at nm. The instructions below are a good guide for what to . Aug 15,  · The indirect assay, usually called a colorimetric assay, takes advantage of a color change of a compound upon binding to the protein. Using the absorbance at nm is the simplest method. The aromatic side chains phenylalanine, tyrosine and tryptophan absorb UV radiation in the range of nm. Colorimetric analysis is based on the change in the intensity of the colour of a solution with variations in concentration. Colorimetric methods represent the simplest form of absorption analysis. The human eye is used to compare the colour of the sample solution with a set of standards until a match is found. Frodo Baggins: A True Hero

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